glosensor camp reporter dna Search Results


glosensor  (ATCC)
98
ATCC glosensor
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor camp dna  (Promega)
90
Promega glosensor camp dna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Camp Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor dna  (Promega)
90
Promega glosensor dna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor camp dna  (Thermo Fisher)
99
Thermo Fisher glosensor camp dna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Camp Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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glosensor-20f  (Promega)
90
Promega glosensor-20f
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor 20f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
glosensor-20f - by Bioz Stars, 2026-05
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glosensor dna  (Thermo Fisher)
99
Thermo Fisher glosensor dna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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glosensor camp-biosensor cdna  (Promega)
90
Promega glosensor camp-biosensor cdna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Camp Biosensor Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor camp-biosensor cdna/product/Promega
Average 90 stars, based on 1 article reviews
glosensor camp-biosensor cdna - by Bioz Stars, 2026-05
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glosensor camp sensor cdna  (Promega)
90
Promega glosensor camp sensor cdna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Camp Sensor Cdna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor camp sensor cdna/product/Promega
Average 90 stars, based on 1 article reviews
glosensor camp sensor cdna - by Bioz Stars, 2026-05
90/100 stars
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glosensor camp reagent  (Promega)
90
Promega glosensor camp reagent
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor Camp Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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glosensor-22f camp plasmid dna  (Promega)
90
Promega glosensor-22f camp plasmid dna
Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of <t>hPTHR1-293</t> cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP <t>GloSensor.</t> Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.
Glosensor 22f Camp Plasmid Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glosensor-22f camp plasmid dna/product/Promega
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glosensor-22f camp plasmid dna - by Bioz Stars, 2026-05
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Image Search Results


Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of hPTHR1-293 cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP GloSensor. Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.

Journal: Cell chemical biology

Article Title: Activity-based, bioorthogonal imaging of phospholipase D reveals spatiotemporal dynamics of GPCR-Gq signaling.

doi: 10.1016/j.chembiol.2021.05.020

Figure Lengend Snippet: Figure 4. Inhibition of PTHR1 endocytosis prolongs PLD activation through Gq and re- duces Gs-cAMP signaling (A) Confocal microscopy of hPTHR1-293 cells treated with rhodamine-PTH (PTH-TMR) for 5– 10 min. Cells were treated with Dyngo-4a or DMSO vehicle 15 min prior to and during ligand addition. Scale bar: 30 mm. (B) Flow cytometry of hPTHR1-293 cells, showing extent of PLD activity over 20 min after the PTH addition ± Dyngo addition. hPTHR1-293 cells were incubated with Dyngo or vehicle for 15 min, stimu- lated with PTH for 0–20 min in the presence of Dyngo or vehicle, followed by addition of oxoTCO (5 min) in the presence of PTH and Dyngo or vehicle, rinsing, addition of Tz-BODIPY, and flow cytometry. The mean BODIPY fluorescence is shown. Gray, back- ground fluorescence in the absence of PTH. (C) Time course of cAMP levels in hPTHR1-293 cells stably expressing cAMP GloSensor. Dyngo or vehicle was added 15 min prior to and during the PTH labeling step. (D) Model indicating competition between Gq and Gs signaling downstream of PTHR1. In (B and C), as- terisks above data denote significance comparing blue and red data points (+ versus Dyngo in the presence of PTH). Error bars represent standard deviation. One-way ANOVA, Games-Howell post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001; n = 3 for (B) and n = 4 for (C). See also Figure S4.

Article Snippet: Stable hPTHR1-HEK 293 expressing GloSensor plasmid originally described in (Maeda et al., 2013) Gs KO-HEK 293 (Gs knockout achieved using CRISPR-Cas9) Laboratory of Thomas Gardella, Harvard Medical School Gs KO-HEK 293 cells, described in (Stallaert et al., 2017) SaOS-2 ATCC Cat#HTB-85, human osteosarcoma SGS-72 ATCC SaOS-2 cells stably expressing GloSensor (Cheloha et al., 2016) UMR-106 ATCC Cat#CRL-1661, Rat osteosarcoma UGS-56 ATCC UMR-106 cells stably expressing GloSensor (Daley et al., 2021) Recombinant DNA hPTHR1-HA plasmid Laboratory of Thomas Gardella, Harvard Medical School, custom plasmid (VectorBuilder).

Techniques: Inhibition, Activation Assay, Confocal Microscopy, Flow Cytometry, Activity Assay, Incubation, Cytometry, Stable Transfection, Expressing, Labeling, Standard Deviation